Dose-Response Effect of Consuming Commercially Available Eggs on Wintertime Serum 25-hydroxyvitamin D Concentrations in Young Australian Adults
Daly M R ,Ross DB ,Gianoudis J ,02 March 2022 ,Dose-Response Effect of Consuming Commercially Available Eggs on Wintertime
Serum 25-hydroxyvitamin D Concentrations in Young Australian Adults: A 12 week
Randomized Controlled Trial ,The Journal of Nutrition ,doi:https://academic.oup.com/jn/advance-article/doi/10.1093/jn/nxac044/6537…
Background: Vitamin D deficiency is a common health concern during winter. Eggs are one
of the few dietary rich sources of vitamin D, containing cholecalciferol (vitamin D3) and 25-
hydroxyvitamin D3 [25(OH)D3], with the latter reported to be five times more potent at
increasing serum 25(OH)D concentrations, the major circulating form of vitamin D. However,
whether there is an optimal dose of eggs to increase or maintain 25(OH)D concentrations
during wintertime is not known.
Objective: To evaluate the dose-response effect of consuming 2, 7 or 12 commercially
available eggs/week on serum 25(OH)D concentrations during the autumn-winter months in
young adults. Secondary aims were to investigate changes in serum lipids, and the feasibility
(adherence) and acceptability to consuming the eggs.
Methods: In a 12-week, randomized controlled trial, 51 adults aged 25-40 years were
randomized to consume 2 eggs/week (control, n=17), 7 eggs/week (n=17) or 12 eggs/week
(n=17). Change in serum 25(OH)D was the primary outcome as assessed by LC/MS/MS.
Serum lipids were assessed using standard techniques and acceptability to consuming the eggs
Results: 42 (82%) participants completed the study. Mean adherence to the eggs was 83% for
controls, 86% for 7 eggs/week and 83% for 12 eggs/week. Mean (95%CI) serum 25(OH)D
concentrations did not change significantly in either the 7 eggs/week [-8.3 (-17.0, 0.4)
nmol/L] or 12 eggs/week [-7.2 (-18.6, 4.3) nmol/L] group, but decreased by 28.6 nmol/L (-
38.1, -18.9) in controls which led to a significant (P=0.003) between-group difference for the
change after 12 weeks. Serum lipids did not differ between the groups, and acceptability
profiles to consuming the eggs were positive and similar for all three groups.